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DNA ligase III antibody [C2C3], C-term

KO/KD Orthogonal
Anti-DNA ligase III antibody [C2C3], C-term used in Western Blot (WB). GTX103197
Anti-DNA ligase III antibody [C2C3], C-term used in Western Blot (WB). GTX103197
Anti-DNA ligase III antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103197

Cat. No. GTX103197

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Hamster
Package
100 μl ($399),
25 μl ($169)
See all DNA ligase III products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036774

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA ligase 3 , LIG2 , LIG3alpha

Cellular Localization

Nucleus

Background

This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNA ligase III antibody [C2C3], C-term used in Western Blot (WB). GTX103197

GTX103197 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [C2C3], C-term (GTX103197) diluted at 1:500.

Anti-DNA ligase III antibody [C2C3], C-term used in Western Blot (WB). GTX103197

GTX103197 WB Image

Orthogonal

HepG2 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [C2C3], C-term (GTX103197) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNA ligase III antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103197

GTX103197 ICC/IF Image

DNA ligase III antibody [C2C3], C-term detects DNA ligase III protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA ligase III protein stained by DNA ligase III antibody [C2C3], C-term (GTX103197) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE

Application Reference

Campalans A et al. Mol Cell Biol 2015; 35 (9) : 1648-58 Interaction with OGG1 is required for efficient recruitment of XRCC1 to base excision repair and maintenance of genetic stability after exposure to oxidative stress.
Application : WB
Reactivity : Hamster
Abdou I et al. Nucleic Acids Res 2015; 43 (2) : 875-92 DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair.
Application : ICC/IF
Reactivity : Human
Truong LN et al. Proc Natl Acad Sci U S A 2013; 110 (19) : 2125834 Microhomology-mediated End Joining and Homologous Recombination share the initial end resection step to repair DNA double-strand breaks in mammalian cells.
Application : WB
Reactivity : Human
Kim S et al. Anticancer Res 2013; 33 (12) : 5445-51 Lobarstin enhances chemosensitivity in human glioblastoma T98G cells.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169