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DNA ligase III antibody [HL2280]

Recombinant antibody KO/KD Orthogonal
Anti-DNA ligase III antibody [HL2280] used in Western Blot (WB). GTX638333
Anti-DNA ligase III antibody [HL2280] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638333
Anti-DNA ligase III antibody [HL2280] used in Western Blot (WB). GTX638333
Anti-DNA ligase III antibody [HL2280] used in Western Blot (WB). GTX638333
Anti-DNA ligase III antibody [HL2280] used in IHC (Paraffin sections) (IHC-P). GTX638333

Cat. No. GTX638333

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2280

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DNA ligase III products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human DNA ligase III.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA ligase 3 , LIG2 , LIG3alpha

Cellular Localization

Mitochondria,Nucleus

Background

This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DNA ligase III antibody [HL2280] used in Western Blot (WB). GTX638333

GTX638333 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNA ligase III antibody [HL2280] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638333

GTX638333 ICC/IF Image

DNA ligase III antibody [HL2280] detects DNA ligase III protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DNA ligase III stained by DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-DNA ligase III antibody [HL2280] used in Western Blot (WB). GTX638333

GTX638333 WB Image

Orthogonal

HepG2 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNA ligase III antibody [HL2280] used in Western Blot (WB). GTX638333

GTX638333 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNA ligase III antibody [HL2280] used in IHC (Paraffin sections) (IHC-P). GTX638333

GTX638333 IHC-P Image

DNA ligase III antibody [HL2280] detects DNA ligase III protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
DNA ligase III stained by DNA ligase III antibody [HL2280] (GTX638333) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations