*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
92/110 kDa. The observed M.W. is based on the following publication. PMID: 9001252
293T , A431 , HeLa , HepG2
Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.01mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human DNA ligase III. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
LIG2 antibody, LIG3 antibody, ligase II, DNA, ATP-dependent antibody, polydeoxyribonucleotide synthase [ATP] 3 antibody, DNA ligase 3 antibody, ligase III, DNA, ATP-dependent antibody
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
GTX103172 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX103172) diluted at 1:500.
GTX103172 ICC/IF Image
DNA ligase III antibody detects LIG3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LIG3 protein stained by DNA ligase III antibody (GTX103172) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX103172 WB Image
DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX103172) diluted by 1:1000.
Observed DNA ligase III protein has mutiple bands at 92 kDa, and 110 kDa, possibly due to alternative splicing.
Reference: PMID: Mol Cell Biol. 1997 Feb;17(2):989-98.
GTX103172 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using DNA ligase 3(GTX103172) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min