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DNA ligase III antibody

KO/KD Orthogonal
Anti-DNA ligase III antibody used in Western Blot (WB). GTX103172
Anti-DNA ligase III antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103172
Anti-DNA ligase III antibody used in Western Blot (WB). GTX103172
Anti-DNA ligase III antibody used in IHC (Paraffin sections) (IHC-P). GTX103172
Anti-DNA ligase III antibody used in Western Blot (WB). GTX103172

Cat No. GTX103172

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all DNA ligase III products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Observed MW

92/110 kDa. The observed M.W. is based on the following publication. PMID: 9001252

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNA ligase III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA ligase 3 , LIG2 , LIG3alpha

Cellular Localization

Nucleus

Background

This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNA ligase III antibody used in Western Blot (WB). GTX103172

GTX103172 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX103172) diluted at 1:500.

Anti-DNA ligase III antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103172

GTX103172 ICC/IF Image

DNA ligase III antibody detects LIG3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LIG3 protein stained by DNA ligase III antibody (GTX103172) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-DNA ligase III antibody used in Western Blot (WB). GTX103172

GTX103172 WB Image

DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX103172) diluted by 1:1000.
Observed DNA ligase III protein has mutiple bands at 92 kDa, and 110 kDa, possibly due to alternative splicing. PMID: 9001252

Anti-DNA ligase III antibody used in IHC (Paraffin sections) (IHC-P). GTX103172

GTX103172 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using DNA ligase 3(GTX103172) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNA ligase III antibody used in Western Blot (WB). GTX103172

GTX103172 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX103172) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Huang Y et al. FASEB J 2018; 6 : fj201800092R Poly(ADP-ribose) polymerase-1 promotes recruitment of meiotic recombination-11 to chromatin and DNA double-strand break repair in Ku70-deficient breast cancer cells.
Application : WB
Reactivity : Human
Howes T. R.L. et al. DNA Repair 2017; : Epub ahead of print Structure-activity relationships among DNA ligase inhibitors; characterization of a selective uncompetitive DNA ligase I inhibitor.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169