GeneTex
United States (US)

DNA ligase III antibody

KO/KD
GTX103172_40135_20160908_WB_shRNA_watermark_19011506_407.jpg
GTX103172_40135_IFA_19011506_752.jpg
GTX103172_40135_WB_19011506_237.jpg
GTX103172_IHC_19011506_417.jpg

Cat No. GTX103172

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Observed MW

92/110 kDa. The observed M.W. is based on the following publication. PMID: 9001252

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNA ligase III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LIG2 antibody, LIG3 antibody, ligase II, DNA, ATP-dependent antibody, polydeoxyribonucleotide synthase [ATP] 3 antibody, DNA ligase 3 antibody, ligase III, DNA, ATP-dependent antibody

Cellular Localization

Nucleus

Background

This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103172_40135_20160908_WB_shRNA_watermark_19011506_407.jpg

GTX103172 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX103172) diluted at 1:500.

GTX103172_40135_IFA_19011506_752.jpg

GTX103172 ICC/IF Image

DNA ligase III antibody detects LIG3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LIG3 protein stained by DNA ligase III antibody (GTX103172) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX103172_40135_WB_19011506_237.jpg

GTX103172 WB Image

DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX103172) diluted by 1:1000.
Observed DNA ligase III protein has mutiple bands at 92 kDa, and 110 kDa, possibly due to alternative splicing.
Reference: PMID: Mol Cell Biol. 1997 Feb;17(2):989-98.

GTX103172_IHC_19011506_417.jpg

GTX103172 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using DNA ligase 3(GTX103172) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169