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DNA ligase IV antibody

Anti-DNA ligase IV antibody used in Western Blot (WB). GTX108820
Anti-DNA ligase IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108820
Anti-DNA ligase IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108820
Anti-DNA ligase IV antibody used in Western Blot (WB). GTX108820
Anti-DNA ligase IV antibody used in Western Blot (WB). GTX108820
Anti-DNA ligase IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108820
Anti-DNA ligase IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108820
Anti-DNA ligase IV antibody used in Western Blot (WB). GTX108820

Cat. No. GTX108820

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DNA ligase IV products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

U87-MG , mouse brain , rat brain

Predict Reactivity

Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase IV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166562

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA ligase 4 , LIG4S

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNA ligase IV antibody used in Western Blot (WB). GTX108820

GTX108820 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (GTX108820) diluted at 1:500.

Anti-DNA ligase IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108820

GTX108820 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA ligase IV(GTX108820) antibody at 1:500 dilution.

Anti-DNA ligase IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108820

GTX108820 ICC/IF Image

DNA ligase IV antibody detects DNA ligase IV protein at nucleus and nucleolus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA ligase IV stained by DNA ligase IV antibody (GTX108820) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DNA ligase IV antibody used in Western Blot (WB). GTX108820

GTX108820 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX108820 diluted at 1:1000

Anti-DNA ligase IV antibody used in Western Blot (WB). GTX108820

GTX108820 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (GTX108820) diluted at 1:500.

Anti-DNA ligase IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108820

GTX108820 IHC-P Image

DNA ligase IV antibody detects DNA ligase IV protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
DNA ligase IV stained by DNA ligase IV antibody (GTX108820) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNA ligase IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108820

GTX108820 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DNA ligase IV(GTX108820) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNA ligase IV antibody used in Western Blot (WB). GTX108820

GTX108820 WB Image

The data was published in the journal EMBO Mol Med in 2015. PMID: 25872942

REFERENCE

Application Reference

Islam A et al. Nucleic Acids Res 2024; 52 (5) : 2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.
Chakraborty A et al. bioRxiv 2023; A glycolytic metabolite restores DNA repair activity of polynucleotide kinase 3'-phosphatase in polyglutamine (PolyQ) diseases.
Chakraborty A et al. J Biol Chem 2023; 299 (3) : 102991 Human DNA polymerase η promotes RNA-templated error-free repair of DNA double-strand breaks.
Chen SH et al. Nucleic Acids Res 2018; (Epub) Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation.
Application : WB
Chakraborty A et al. Nat Commun 2016; 7 : 13049 Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169