GeneTex
United States (US)

DNA ligase IV antibody

GTX108820_40506_20160331_WB_R_brain_19011507_106.jpg
GTX108820_40506_20160331_WB_M_brain_19011507_524.jpg
GTX108820_40506_IHC_19011507_833.jpg
GTX108820_40506_WB_19011507_462.jpg
GTX108820_40506_IFA_19011507_710.jpg

Cat No. GTX108820

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

U87-MG , mouse brain , rat brain

Predict Reactivity

Sheep, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase IV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Ligase 4,Lig4S,Lig4

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108820_40506_20160331_WB_R_brain_19011507_106.jpg

GTX108820 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (GTX108820) diluted at 1:500.

GTX108820_40506_20160331_WB_M_brain_19011507_524.jpg

GTX108820 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (GTX108820) diluted at 1:500.

GTX108820_40506_IHC_19011507_833.jpg

GTX108820 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DNA ligase IV(GTX108820) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108820_40506_WB_19011507_462.jpg

GTX108820 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX108820 diluted at 1:1000

GTX108820_40506_IFA_19011507_710.jpg

GTX108820 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA ligase IV(GTX108820) antibody at 1:500 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169