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DNA ligase IV antibody

Anti-DNA ligase IV antibody used in Western Blot (WB). GTX55592
Anti-DNA ligase IV antibody used in IHC (Paraffin sections) (IHC-P). GTX55592

Cat. No. GTX55592

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ChIP assay

Reactivity

 Human
Package
100 μl ($399)
See all DNA ligase IV products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human LIG4 (NP_002303.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887931

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA ligase 4 , LIG4S

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DNA ligase IV antibody used in Western Blot (WB). GTX55592

GTX55592 WB Image

WB analysis of PC-3 whole cell lysate using GTX55592 DNA ligase IV antibody.
Dilution : 1:1000
Loading : 25μg

Anti-DNA ligase IV antibody used in IHC (Paraffin sections) (IHC-P). GTX55592

GTX55592 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX55592 DNA ligase IV antibody.
Antigen retrieval : High pressure antigen retrieval with 10 mM citrate buffer pH 6.0
Dilution : 1:100

REFERENCE

Application Reference

PE Head et al. Nucleic Acids Res 2023; : gkad549 DNA-PK is activated by SIRT2 deacetylation to promote DNA double-strand break repair by non-homologous end joining
Application : ChIP assay
Reactivity : Human
Ruben Schep et al. Mol Cell 2021; 81 (10) : 2216-2230.e10 Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance.
Application : ChIP assay
Reactivity : Human
Clouaire T et al. Mol Cell 2018; 72 (2) : 250-262.e6 Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures.
Application : ChIP assay
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399