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DNA polymerase beta antibody [GT1251]

Recombinant antibody Orthogonal
Anti-DNA polymerase beta antibody [GT1251] used in Western Blot (WB). GTX02848
Anti-DNA polymerase beta antibody [GT1251] used in Western Blot (WB). GTX02848
Anti-DNA polymerase beta antibody [GT1251] used in Western Blot (WB). GTX02848
Anti-DNA polymerase beta antibody [GT1251] used in IHC (Paraffin sections) (IHC-P). GTX02848
Anti-DNA polymerase beta antibody [GT1251] used in IHC (Paraffin sections) (IHC-P). GTX02848
Anti-DNA polymerase beta antibody [GT1251] used in Western Blot (WB). GTX02848

Cat. No. GTX02848

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1251

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all DNA polymerase beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human DNA Polymerase beta

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase beta

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase beta antibody [GT1251] used in Western Blot (WB). GTX02848

GTX02848 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [GT1251] (GTX02848) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNA polymerase beta antibody [GT1251] used in Western Blot (WB). GTX02848

GTX02848 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [GT1251] (GTX02848) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNA polymerase beta antibody [GT1251] used in Western Blot (WB). GTX02848

GTX02848 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [GT1251] (GTX02848) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-DNA polymerase beta antibody [GT1251] used in IHC (Paraffin sections) (IHC-P). GTX02848

GTX02848 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX02848 DNA polymerase beta antibody [GT1251].
Dilution : 1:100

Anti-DNA polymerase beta antibody [GT1251] used in IHC (Paraffin sections) (IHC-P). GTX02848

GTX02848 IHC-P Image

IHC-P analysis of rat testis tissue section using GTX02848 DNA polymerase beta antibody [GT1251].
Dilution : 1:100

Anti-DNA polymerase beta antibody [GT1251] used in Western Blot (WB). GTX02848

GTX02848 WB Image

WB analysis of various samples using GTX02848 DNA polymerase beta antibody [GT1251].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 419