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DNA polymerase beta antibody [HL1512]

Recombinant antibody KO/KD
Anti-DNA polymerase beta antibody [HL1512] used in Western Blot (WB). GTX636986
Anti-DNA polymerase beta antibody [HL1512] used in IHC (Paraffin sections) (IHC-P). GTX636986
Anti-DNA polymerase beta antibody [HL1512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636986
Anti-DNA polymerase beta antibody [HL1512] used in Western Blot (WB). GTX636986
Anti-DNA polymerase beta antibody [HL1512] used in IHC (Paraffin sections) (IHC-P). GTX636986
Anti-DNA polymerase beta antibody [HL1512] used in Western Blot (WB). GTX636986
Anti-DNA polymerase beta antibody [HL1512] used in Western Blot (WB). GTX636986

Cat. No. GTX636986

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1512

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all DNA polymerase beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNA polymerase beta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase beta

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase beta antibody [HL1512] used in Western Blot (WB). GTX636986

GTX636986 WB Image

KO/KD

Wild-type (WT) and POLB knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-DNA polymerase beta antibody [HL1512] used in IHC (Paraffin sections) (IHC-P). GTX636986

GTX636986 IHC-P Image

DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNA polymerase beta antibody [HL1512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636986

GTX636986 ICC/IF Image

DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 10 min.
Green: DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-DNA polymerase beta antibody [HL1512] used in Western Blot (WB). GTX636986

GTX636986 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNA polymerase beta antibody [HL1512] used in IHC (Paraffin sections) (IHC-P). GTX636986

GTX636986 IHC-P Image

DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNA polymerase beta antibody [HL1512] used in Western Blot (WB). GTX636986

GTX636986 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNA polymerase beta antibody [HL1512] used in Western Blot (WB). GTX636986

GTX636986 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations