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DNA polymerase beta antibody [HL1525]

Recombinant antibody Orthogonal
Anti-DNA polymerase beta antibody [HL1525] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636999
Anti-DNA polymerase beta antibody [HL1525] used in Western Blot (WB). GTX636999

Cat. No. GTX636999

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1525

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all DNA polymerase beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase beta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase beta

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase beta antibody [HL1525] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636999

GTX636999 ICC/IF Image

Orthogonal

DNA polymerase beta antibody [HL1525] detects DNA polymerase beta protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA polymerase beta stained by DNA polymerase beta antibody [HL1525] (GTX636999) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DNA polymerase beta antibody [HL1525] used in Western Blot (WB). GTX636999

GTX636999 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1525] (GTX636999) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations