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DNA polymerase beta antibody

KO/KD
Anti-DNA polymerase beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55594
Anti-DNA polymerase beta antibody used in IHC (Paraffin sections) (IHC-P). GTX55594
Anti-DNA polymerase beta antibody used in Western Blot (WB). GTX55594
Anti-DNA polymerase beta antibody used in Western Blot (WB). GTX55594
Anti-DNA polymerase beta antibody used in IHC (Paraffin sections) (IHC-P). GTX55594

Cat. No. GTX55594

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all DNA polymerase beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200 - 1:1000
ICC/IF 1:20 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human POLB (NP_002681.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase beta

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55594

GTX55594 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55594 DNA polymerase beta antibody.
Blue : DAPI

Anti-DNA polymerase beta antibody used in IHC (Paraffin sections) (IHC-P). GTX55594

GTX55594 IHC-P Image

IHC-P analysis of human stomach tissue using GTX55594 DNA polymerase beta antibody.
Dilution : 1:100

Anti-DNA polymerase beta antibody used in Western Blot (WB). GTX55594

GTX55594 WB Image

WB analysis of various sample lysates using GTX55594 DNA polymerase beta antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DNA polymerase beta antibody used in Western Blot (WB). GTX55594

GTX55594 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55594 DNA polymerase beta antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DNA polymerase beta antibody used in IHC (Paraffin sections) (IHC-P). GTX55594

GTX55594 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX55594 DNA polymerase beta antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399