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DNA polymerase delta antibody [6196]

Anti-DNA polymerase delta antibody [6196] used in Western Blot (WB). GTX77606
Anti-DNA polymerase delta antibody [6196] used in IHC (Paraffin sections) (IHC-P). GTX77606

Cat. No. GTX77606

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6196

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all DNA polymerase delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encoding amino acids 1-140 of the large subunit of human DNA polymerase d.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_424560

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase delta 1, catalytic subunit , CDC2 , CRCS10 , MDPL , POLD

Cellular Localization

Nucleus

Background

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase delta antibody [6196] used in Western Blot (WB). GTX77606

GTX77606 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase delta antibody [6196] (GTX77606) diluted at 1:1000.

Anti-DNA polymerase delta antibody [6196] used in IHC (Paraffin sections) (IHC-P). GTX77606

GTX77606 IHC-P Image

DNA polymerase delta antibody [6196] detects DNA polymerase delta protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
DNA polymerase delta stained by DNA polymerase delta antibody [6196] (GTX77606) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations