GeneTex
United States (US)

DNA polymerase delta antibody

GTX102888_39939_20180504_WB_19011506_745.jpg
GTX102888_39939_20141128_IHC_19011506_527.jpg
GTX102888_39939_WB_R_19011506_202.jpg
GTX102888_WB_19011506_332.jpg
GTX102888_IFA_19011506_808.jpg

Cat No. GTX102888

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Molt-4 , Rat2

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNA polymerase delta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Polymerase Delta 1, Catalytic Subunit,Cdc2,Crcs10,Mdpl,Pold,Pold1

Cellular Localization

Nucleus

Background

The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1, POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102888_39939_20180504_WB_19011506_745.jpg

GTX102888 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase delta antibody (GTX102888) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102888_39939_20141128_IHC_19011506_527.jpg

GTX102888 IHC-P Image

DNA polymerase delta antibody detects DNA polymerase delta protein at cytoplasm and nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
DNA polymerase delta antibody (GTX102888) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102888_39939_WB_R_19011506_202.jpg

GTX102888 WB Image

DNA polymerase delta antibody detects POLD1 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
5 % SDS-PAGE
DNA polymerase delta antibody (GTX102888) dilution: 1:1000

GTX102888_WB_19011506_332.jpg

GTX102888 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX102888 diluted at 1:3000

GTX102888_IFA_19011506_808.jpg

GTX102888 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA Polymerase delta, catalytic subunit(GTX102888) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169