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DNA polymerase delta antibody

Anti-DNA polymerase delta antibody used in Western Blot (WB). GTX64730
Anti-DNA polymerase delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64730
Anti-DNA polymerase delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64730
Anti-DNA polymerase delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64730

Cat. No. GTX64730

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all DNA polymerase delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 808-1107 of human POLD1 (NP_002682.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase delta 1, catalytic subunit , CDC2 , CRCS10 , MDPL , POLD

Cellular Localization

Nucleus

Background

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase delta antibody used in Western Blot (WB). GTX64730

GTX64730 WB Image

WB analysis of various sample lysates using GTX64730 DNA polymerase delta antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DNA polymerase delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64730

GTX64730 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64730 DNA polymerase delta antibody.
Blue : DAPI

Anti-DNA polymerase delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64730

GTX64730 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64730 DNA polymerase delta antibody. Cells were treated by UV-A laser.
Red : Primary antibody
Green : gamma H2AX (phospho S139) used for DNA damage marker
Blue : DAPI

Anti-DNA polymerase delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64730

GTX64730 ICC/IF Image

ICC/IF analysis of A549 cells using GTX64730 DNA polymerase delta antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399