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DNA polymerase epsilon 3 antibody [N1C3]

KO/KD
Anti-DNA polymerase epsilon 3 antibody [N1C3] used in Western Blot (WB). GTX116557
Anti-DNA polymerase epsilon 3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116557
Anti-DNA polymerase epsilon 3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116557
Anti-DNA polymerase epsilon 3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116557
Anti-DNA polymerase epsilon 3 antibody [N1C3] used in Western Blot (WB). GTX116557

Cat. No. GTX116557

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DNA polymerase epsilon 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNA polymerase epsilon 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624395

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase epsilon 3, accessory subunit , CHARAC17 , CHRAC17 , CHRAC2 , YBL1 , p17

Background

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase epsilon 3 antibody [N1C3] used in Western Blot (WB). GTX116557

GTX116557 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with DNA polymerase epsilon 3 antibody [N1C3] (GTX116557) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-DNA polymerase epsilon 3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116557

GTX116557 IHC-P Image

DNA polymerase epsilon antibody [N1C3] detects DNA Polymerase epsilon protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
DNA Polymerase epsilon antibody [N1C3] (GTX116557) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNA polymerase epsilon 3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116557

GTX116557 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DNA polymerase epsilon(GTX116557) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNA polymerase epsilon 3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116557

GTX116557 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA polymerase epsilon(GTX116557) antibody at 1:200 dilution.

Anti-DNA polymerase epsilon 3 antibody [N1C3] used in Western Blot (WB). GTX116557

GTX116557 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
15% SDS PAGE
GTX116557 diluted at 1:1000

REFERENCE

Application Reference

Zou W et al. J Virol 2017; (Epub) Human Parvovirus B19 Utilizes Cellular DNA Replication Machinery for Viral DNA Replication.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169