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DNA polymerase epsilon 3 antibody

Anti-DNA polymerase epsilon 3 antibody used in Western Blot (WB). GTX129526
Anti-DNA polymerase epsilon 3 antibody used in Western Blot (WB). GTX129526

Cat No. GTX129526

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , POLE3 shRNA-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNA polymerase epsilon 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA polymerase epsilon 3, accessory subunit , CHARAC17 , CHRAC17 , CHRAC2 , YBL1 , p17

Cellular Localization

Nucleus

Background

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase epsilon 3 antibody used in Western Blot (WB). GTX129526

GTX129526 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with DNA polymerase epsilon 3 antibody (GTX129526) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-DNA polymerase epsilon 3 antibody used in Western Blot (WB). GTX129526

GTX129526 WB Image

DNA polymerase epsilon antibody detects DNA polymerase epsilon protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
15 % SDS-PAGE
DNA polymerase epsilon antibody (GTX129526) dilution: 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf