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DNA polymerase epsilon 3 antibody

KO/KD
Anti-DNA polymerase epsilon 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33158
Anti-DNA polymerase epsilon 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33158
Anti-DNA polymerase epsilon 3 antibody used in Western Blot (WB). GTX33158
Anti-DNA polymerase epsilon 3 antibody used in Western Blot (WB). GTX33158

Cat. No. GTX33158

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all DNA polymerase epsilon 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase epsilon 3, accessory subunit , CHARAC17 , CHRAC17 , CHRAC2 , YBL1 , p17

Cellular Localization

Nucleus

Background

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase epsilon 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33158

GTX33158 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33158 DNA polymerase epsilon 3 antibody. Cells were treated by UV-A laser.
Red : Primary antibody
Green : gamma H2AX (phospho S139) used for DNA damage marker
Blue : DAPI

Anti-DNA polymerase epsilon 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33158

GTX33158 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33158 DNA polymerase epsilon 3 antibody.

Anti-DNA polymerase epsilon 3 antibody used in Western Blot (WB). GTX33158

GTX33158 WB Image

WB analysis of various sample lysates using GTX33158 DNA polymerase epsilon 3 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DNA polymerase epsilon 3 antibody used in Western Blot (WB). GTX33158

GTX33158 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX33158 DNA polymerase epsilon 3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399