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DNA polymerase gamma antibody [N1N2], N-term

Anti-DNA polymerase gamma antibody [N1N2], N-term used in Western Blot (WB). GTX113247
Anti-DNA polymerase gamma antibody [N1N2], N-term used in Western Blot (WB). GTX113247
Anti-DNA polymerase gamma antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113247

Cat. No. GTX113247

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all DNA polymerase gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

Positive Control

NT2D1 , rat testis

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DNA polymerase gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732234

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase gamma, catalytic subunit , MDP1 , MIRAS , MTDPS4A , MTDPS4B , PEO , POLG1 , POLGA , SANDO , SCAE

Cellular Localization

Mitochondrion

Background

Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNA polymerase gamma antibody [N1N2], N-term used in Western Blot (WB). GTX113247

GTX113247 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
5% SDS PAGE
GTX113247 diluted at 1:1000

Anti-DNA polymerase gamma antibody [N1N2], N-term used in Western Blot (WB). GTX113247

GTX113247 WB Image

DNA polymerase gamma antibody [N1N2], N-term detects POLG protein by Western blot analysis.
A. 50 μg rat testis lysate/extract
5 % SDS-PAGE
DNA polymerase gamma antibody [N1N2], N-term (GTX113247) dilution: 1:500

Anti-DNA polymerase gamma antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113247

GTX113247 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using DNA polymerase gamma(GTX113247) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169