APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Rat testis , MCF-7 , MDA-MB-231
Predict Reactivity
Mouse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.67 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase gamma. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240719
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DNA polymerase gamma, catalytic subunit , MDP1 , MIRAS , MTDPS4A , MTDPS4B , PEO , POLG1 , POLGA , SANDO , SCAE
Cellular Localization
Mitochondrion
Background
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100398 IP Image
Immunoprecipitation of DNA polymerase gamma protein from MCF-7 whole cell extracts using 5 μg of DNA polymerase gamma antibody (GTX100398).
Western blot analysis was performed using DNA polymerase gamma antibody (GTX100398).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX100398 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using DNA polymerase gamma(GTX100398) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100398 WB Image
DNA polymerase gamma antibody detects DNA polymerase gamma protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase gamma antibody (GTX100398) diluted by 1:1000.
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GTX100398 WB Image
DNA polymerase gamma antibody detects POLG protein by western blot analysis.
A. 50 μg Rat testis lysate/extract
5% SDS-PAGE
DNA polymerase gamma antibody (GTX100398) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100398 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma(GTX100398) antibody at 1:200 dilution.
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REFERENCE
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REVIEW
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