GeneTex
United States (US)

DNA polymerase gamma antibody

GTX100398_39443_IFA_19011506_165.jpg
GTX100398_40247_WB_R_testis_19011506_628.jpg
GTX100398_41822_WB_19011506_809.jpg
GTX100398_39443_IHC_19011506_172.jpg
GTX100398_40247_20150317_IP_19011506_970.jpg

Cat No. GTX100398

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231 , Rat testis

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Polymerase Gamma, Catalytic Subunit,Mdp1,Miras,Mtdps4A,Mtdps4B,Peo,Polg1,Polga,Sando,Scae,Polg

Cellular Localization

Mitochondrion

Background

Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100398_39443_IFA_19011506_165.jpg

GTX100398 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma(GTX100398) antibody at 1:200 dilution.

GTX100398_40247_WB_R_testis_19011506_628.jpg

GTX100398 WB Image

DNA polymerase gamma antibody detects POLG protein by western blot analysis.
A. 50 µg Rat testis lysate/extract
5% SDS-PAGE
DNA polymerase gamma antibody (GTX100398) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100398_41822_WB_19011506_809.jpg

GTX100398 WB Image

DNA polymerase gamma antibody detects DNA polymerase gamma protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase gamma antibody (GTX100398) diluted by 1:1000.

GTX100398_39443_IHC_19011506_172.jpg

GTX100398 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using DNA polymerase gamma(GTX100398) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100398_40247_20150317_IP_19011506_970.jpg

GTX100398 IP Image

Immunoprecipitation of DNA polymerase gamma protein from MCF-7 whole cell extracts using 5 μg of DNA polymerase gamma antibody (GTX100398).
Western blot analysis was performed using DNA polymerase gamma antibody (GTX100398).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169