DNA polymerase iota antibody

Name: DNA polymerase iota antibody
Brand: GeneTex
SKU: GTX112137
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-DNA polymerase iota antibody used in Western Blot (WB). GTX112137

Cat. No. GTX112137

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all DNA polymerase iota products

View product citations for antibody GTX112137 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNA polymerase iota. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623598

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase iota , RAD30B , RAD3OB , eta2

Cellular Localization

Nucleus

Background

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Database

Gene ID: 11201 POLI
UniProt: Q9UNA4 POLI

Research Area

DATA IMAGES

GTX112137 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
7.5% SDS PAGE
GTX112137 diluted at 1:500

GTX112137 WB Image

The data was published in the 2021 in J Biol Chem. PMID: 33753168

REFERENCE

Application Reference

Rebekah J Hutcherson et al. J Biol Chem 2021; : 100570 Age and insulin-like growth factor-1 impact PCNA monoubiquitination in UVB-irradiated human skin.

Application : WB

Reactivity : Human

Deng X et al. PLoS Pathog 2016; 12 (1) : e1005399 Replication of an Autonomous Human Parvovirus in Non-dividing Human Airway Epithelium Is Facilitated through the DNA Damage and Repair Pathways.

Application : WB

Reactivity : Human

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