GeneTex
United States (US)

DNA polymerase iota antibody

GTX30135_181_ICC-IF_18121410_329.jpg
GTX30135_1340_WB_18121410_357.jpg

Cat No. GTX30135

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:250
Not tested in other applications.

Calculated MW

83 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Serum, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the C-terminal region of human DNA Polymerase iota between amino acids 700-740, conjugated to KLH. [UniProt# Q9UNA4]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RAD30B antibody , RAD3OB antibody , eta2 antibody

Cellular Localization

Nucleus

Background

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Database

DATA IMAGES
GTX30135_181_ICC-IF_18121410_329.jpg

GTX30135 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30135 DNA polymerase iota antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:250

GTX30135_1340_WB_18121410_357.jpg

GTX30135 WB Image

WB analysis of NIH-3T3 cell lysate using GTX30135 DNA polymerase iota antibody.

Package List Price ($)
$ 329