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DNAJA2 antibody [3A1]

Overexpression
Anti-DNAJA2 antibody [3A1] used in Western Blot (WB). GTX84610
Anti-DNAJA2 antibody [3A1] used in Flow cytometry (FACS). GTX84610
Anti-DNAJA2 antibody [3A1] used in Western Blot (WB). GTX84610
Anti-DNAJA2 antibody [3A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84610
Anti-DNAJA2 antibody [3A1] used in IHC (Paraffin sections) (IHC-P). GTX84610
Anti-DNAJA2 antibody [3A1] used in Western Blot (WB). GTX84610
Anti-DNAJA2 antibody [3A1] used in IHC (Paraffin sections) (IHC-P). GTX84610
Anti-DNAJA2 antibody [3A1] used in IHC (Paraffin sections) (IHC-P). GTX84610

Cat. No. GTX84610

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A1

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($489)
See all DNAJA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human DNAJA2 (NP_005871) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10730420

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member A2 , CPR3 , DJ3 , DJA2 , DNAJ , DNJ3 , HIRIP4 , PRO3015 , RDJ2

Cellular Localization

Membrane

Background

Co-chaperone of Hsc70.

Database

Research Area

DATA IMAGES

Anti-DNAJA2 antibody [3A1] used in Western Blot (WB). GTX84610

GTX84610 WB Image

Overexpression

WB analysis of HEK293T cells transfected with DNAJA2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84610 DNAJA2 antibody [3A1].
Loading : 5 ug per lane

Anti-DNAJA2 antibody [3A1] used in Flow cytometry (FACS). GTX84610

GTX84610 FACS Image

FACS analysis of HEK293T cells transfected with either DNAJA2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84610 DNAJA2 antibody [3A1].

Anti-DNAJA2 antibody [3A1] used in Western Blot (WB). GTX84610

GTX84610 WB Image

WB analysis of various cell lines using GTX84610 DNAJA2 antibody [3A1].
Loading : 35 ug per lane

Anti-DNAJA2 antibody [3A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84610

GTX84610 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with DNAJA2 plasmid using GTX84610 DNAJA2 antibody [3A1].

Anti-DNAJA2 antibody [3A1] used in IHC (Paraffin sections) (IHC-P). GTX84610

GTX84610 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX84610 DNAJA2 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-DNAJA2 antibody [3A1] used in Western Blot (WB). GTX84610

GTX84610 WB Image

WB analysis of various samples using GTX84610 DNAJA2 antibody [3A1].
Loading : 10 ug per lane
Dilution : 1:200

Anti-DNAJA2 antibody [3A1] used in IHC (Paraffin sections) (IHC-P). GTX84610

GTX84610 IHC-P Image

IHC-P analysis of human colon tissue using GTX84610 DNAJA2 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-DNAJA2 antibody [3A1] used in IHC (Paraffin sections) (IHC-P). GTX84610

GTX84610 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX84610 DNAJA2 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

Application Reference

Vonk WIM et al. Mol Cell 2020; 78 (2) : 329-345.e9 Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489