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DNAJA2 antibody [N1C2]

Anti-DNAJA2 antibody [N1C2] used in Western Blot (WB). GTX115393
Anti-DNAJA2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115393
Anti-DNAJA2 antibody [N1C2] used in Western Blot (WB). GTX115393

Cat. No. GTX115393

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all DNAJA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621411

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member A2 , CPR3 , DJ3 , DJA2 , DNAJ , DNJ3 , HIRIP4 , PRO3015 , RDJ2

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNAJA2 antibody [N1C2] used in Western Blot (WB). GTX115393

GTX115393 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX115393 diluted at 1:500

Anti-DNAJA2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115393

GTX115393 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using DNAJA2 antibody [N1C2] (GTX115393) at 1:300 dilution.

Anti-DNAJA2 antibody [N1C2] used in Western Blot (WB). GTX115393

GTX115393 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
B: zebrafish brain
10% SDS PAGE
GTX115393 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169