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DNAJA2 antibody

Anti-DNAJA2 antibody used in Flow cytometry (FACS). GTX04359
Anti-DNAJA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04359
Anti-DNAJA2 antibody used in Flow cytometry (FACS). GTX04359
Anti-DNAJA2 antibody used in Flow cytometry (FACS). GTX04359
Anti-DNAJA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04359
Anti-DNAJA2 antibody used in Western Blot (WB). GTX04359
Anti-DNAJA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04359
Anti-DNAJA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04359

Cat. No. GTX04359

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($469)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5μg/ml
IHC-P 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cells
ELISA 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human DNAJA2 recombinant protein (Position: M1-N140).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member A2 , CPR3 , DJ3 , DJA2 , DNAJ , DNJ3 , HIRIP4 , PRO3015 , RDJ2

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DNAJA2 antibody used in Flow cytometry (FACS). GTX04359

GTX04359 FACS Image

FACS analysis of NRK cells using GTX04359 DNAJA2 antibody.
Blue line : Primary antibody
Green line : Isotype control
Red line : Unlabelled cell
Antibody amount : 1μg/1x10⁶ cells

Anti-DNAJA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04359

GTX04359 IHC-P Image

IHC-P analysis of human testicular cancer tissue using GTX04359 DNAJA2 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0).
Dilution : 1μg/ml

Anti-DNAJA2 antibody used in Flow cytometry (FACS). GTX04359

GTX04359 FACS Image

FACS analysis of A431 cells using GTX04359 DNAJA2 antibody.
Blue line : Primary antibody
Green line : Isotype control
Red line : Unlabelled cell
Antibody amount : 1μg/1x10⁶ cells

Anti-DNAJA2 antibody used in Flow cytometry (FACS). GTX04359

GTX04359 FACS Image

FACS analysis of ANA-1 cells using GTX04359 DNAJA2 antibody.
Blue line : Primary antibody
Green line : Isotype control
Red line : Unlabelled cell
Antibody amount : 1μg/1x10⁶ cells

Anti-DNAJA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04359

GTX04359 IHC-P Image

IHC-P analysis of human rectal cancer tissue using GTX04359 DNAJA2 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0).
Dilution : 1μg/ml

Anti-DNAJA2 antibody used in Western Blot (WB). GTX04359

GTX04359 WB Image

WB analysis of various sample lysates using GTX04359 DNAJA2 antibody.
Lane 1 : Jurkat whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : Raji whole cell lysate
Lane 5 : rat kidney tissue lysate
Lane 6 : mouse testis tissue lysate
Lane 7 : NIH-3T3 whole cell lysate
Dilution : 0.5 μg/mL
Loading : 50 μg

Anti-DNAJA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04359

GTX04359 IHC-P Image

IHC-P analysis of rat testis tissue using GTX04359 DNAJA2 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0).
Dilution : 1μg/ml

Anti-DNAJA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04359

GTX04359 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX04359 DNAJA2 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0).
Dilution : 1μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 469