APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Sheep, Bovine, Chicken, Pig, Horse, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human DNAJA2(Accession O60884), corresponding to amino acid residues I294-N344.
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DnaJ heat shock protein family (Hsp40) member A2 , CPR3 , DJ3 , DJA2 , DNAJ , DNJ3 , HIRIP4 , PRO3015 , RDJ2
Cellular Localization
Membrane
Background
The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04360 WB Image
WB analysis of various sample lysates using GTX04360 DNAJA2 antibody.
Lane 1: HeLa whole cell lysate with Blocking pepetide treatment
Lane 2: HeLa whole cell lysate
Lane 3: B16F10 whole cell lysate
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GTX04360 WB Image
WB analysis of A375 cells lysates using GTX04360 DNAJA2 antibody. The lane on the left was treated with blocking peptide.
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GTX04360 ICC/IF Image
ICC/IF analysis of PFA fixed HeLa cells using GTX04360 DNAJA2 antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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REFERENCE
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REVIEW
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