Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNAJB5 antibody (GTX119124) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DNAJB5 antibody detects DNAJB5 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
DNAJB5 antibody (GTX119124) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min