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DNAJB6 antibody [N3C3]

Anti-DNAJB6 antibody [N3C3] used in Western Blot (WB). GTX101947
Anti-DNAJB6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101947
Anti-DNAJB6 antibody [N3C3] used in Western Blot (WB). GTX101947

Cat No. GTX101947

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Molt-4 , MDA-MB-231

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJB6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165566

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member B6 , DJ4 , DnaJ , HHDJ1 , HSJ-2 , HSJ2 , LGMD1D , LGMD1E , LGMDD1 , MRJ , MSJ-1

Cellular Localization

Cytoplasm , perinuclear region , Nucleus

Background

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNAJB6 antibody [N3C3] used in Western Blot (WB). GTX101947

GTX101947 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNAJB6 antibody [N3C3] (GTX101947) diluted at 1:500.

Anti-DNAJB6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101947

GTX101947 ICC/IF Image

DNAJB6 antibody [N3C3] detects DNAJB6 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNAJB6 protein stained by DNAJB6 antibody [N3C3] (GTX101947) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-DNAJB6 antibody [N3C3] used in Western Blot (WB). GTX101947

GTX101947 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNAJB6 antibody [N3C3] (GTX101947) diluted at 1:500.

REFERENCE

REVIEW

DNAJB6 antibody [N3C3] Cat No. GTX101947

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2012. PMID: 23133382
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169