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DNAJB6 antibody [N3C3]

Anti-DNAJB6 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX101947
Anti-DNAJB6 antibody [N3C3] used in Western Blot (WB). GTX101947
Anti-DNAJB6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101947
Anti-DNAJB6 antibody [N3C3] used in Western Blot (WB). GTX101947
Anti-DNAJB6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101947

Cat. No. GTX101947

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all DNAJB6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Molt-4 , MDA-MB-231

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJB6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165566

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member B6 , DJ4 , DnaJ , HHDJ1 , HSJ-2 , HSJ2 , LGMD1D , LGMD1E , LGMDD1 , MRJ , MSJ-1

Cellular Localization

Cytoplasm , perinuclear region , Nucleus

Background

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNAJB6 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX101947

GTX101947 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using DNAJB6 antibody [N3C3] (GTX101947) at 1:200 dilution.

Anti-DNAJB6 antibody [N3C3] used in Western Blot (WB). GTX101947

GTX101947 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNAJB6 antibody [N3C3] (GTX101947) diluted at 1:500.

Anti-DNAJB6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101947

GTX101947 ICC/IF Image

DNAJB6 antibody [N3C3] detects DNAJB6 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNAJB6 protein stained by DNAJB6 antibody [N3C3] (GTX101947) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-DNAJB6 antibody [N3C3] used in Western Blot (WB). GTX101947

GTX101947 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNAJB6 antibody [N3C3] (GTX101947) diluted at 1:500.

Anti-DNAJB6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101947

GTX101947 ICC/IF Image

The data was published in the journal PLoS Pathog in 2012. PMID: 23133382

REFERENCE

Application Reference

Yonggang Pei et al. PLoS Pathog 2012; 8 (11) : e1002968 A Hsp40 chaperone protein interacts with and modulates the cellular distribution of the primase protein of human cytomegalovirus.
Application : ICC/IF
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169