GeneTex
United States (US)

DNAJB6 antibody [N3C3]

GTX101947_40520_20161117_WB_2_19011506_167.jpg
GTX101947_40520_IFA_19011506_203.jpg
GTX101947_40520_20161117_WB_19011506_516.jpg

Cat No. GTX101947

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Molt-4 , MDA-MB-231

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJB6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dnaj Heat Shock Protein Family (Hsp40) Member B6,Dj4,Dnaj,Hhdj1,Hsj-2,Hsj2,Lgmd1D,Lgmd1E,Mrj,Msj-1,Dnajb6

Cellular Localization

Cytoplasm , perinuclear region , Nucleus

Background

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101947_40520_20161117_WB_2_19011506_167.jpg

GTX101947 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNAJB6 antibody [N3C3] (GTX101947) diluted at 1:500.

GTX101947_40520_IFA_19011506_203.jpg

GTX101947 ICC/IF Image

DNAJB6 antibody [N3C3] detects DNAJB6 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNAJB6 protein stained by DNAJB6 antibody [N3C3] (GTX101947) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101947_40520_20161117_WB_19011506_516.jpg

GTX101947 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNAJB6 antibody [N3C3] (GTX101947) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf