GeneTex
United States (US)

DNAJB6 antibody

GTX101946_IFA_19011506_381.jpg
GTX101946_39820_20161117_WB_19011506_466.jpg

Cat No. GTX101946

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

MDA-MB-231

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJB6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DJ4 antibody, DKFZp566D0824 antibody, DnaJ antibody, FLJ42837 antibody, HHDJ1 antibody, HSJ-2 antibody, HSJ2 antibody, MGC1152 antibody, MGC117297 antibody, MRJ antibody, MSJ-1 antibody, DNAJB6 antibody, heat shock protein J2 antibody, dnaJ homolog subfamily B member 6 antibody, DnaJ-like 2 protein antibody, "DnaJ (Hsp40) homolog, subfamily B, member 6 antibody"

Cellular Localization

Cytoplasm , perinuclear region , Nucleus

Background

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101946_IFA_19011506_381.jpg

GTX101946 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNAJB6(GTX101946) antibody at 1:200 dilution.

GTX101946_39820_20161117_WB_19011506_466.jpg

GTX101946 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNAJB6 antibody (GTX101946) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf