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DNAJB6 antibody

Orthogonal
Anti-DNAJB6 antibody used in Western Blot (WB). GTX33160
Anti-DNAJB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33160
Anti-DNAJB6 antibody used in IHC (Paraffin sections) (IHC-P). GTX33160
Anti-DNAJB6 antibody used in IHC (Paraffin sections) (IHC-P). GTX33160
Anti-DNAJB6 antibody used in Western Blot (WB). GTX33160

Cat. No. GTX33160

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DNAJB6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887699

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member B6 , DJ4 , DnaJ , HHDJ1 , HSJ-2 , HSJ2 , LGMD1D , LGMD1E , LGMDD1 , MRJ , MSJ-1

Cellular Localization

Nucleus

Background

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DNAJB6 antibody used in Western Blot (WB). GTX33160

GTX33160 WB Image

WB analysis of various sample lysates using GTX33160 DNAJB6 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DNAJB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33160

GTX33160 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33160 DNAJB6 antibody.
Blue : DAPI

Anti-DNAJB6 antibody used in IHC (Paraffin sections) (IHC-P). GTX33160

GTX33160 IHC-P Image

IHC-P analysis of mouse ileum tissue using GTX33160 DNAJB6 antibody.
Dilution : 1:100

Anti-DNAJB6 antibody used in IHC (Paraffin sections) (IHC-P). GTX33160

GTX33160 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX33160 DNAJB6 antibody.
Dilution : 1:100

Anti-DNAJB6 antibody used in Western Blot (WB). GTX33160

GTX33160 WB Image

Orthogonal

The data was published in the journal Front Neurol in 2019.PMID: 31258504

REFERENCE

Application Reference

Gatz C et al. Front Neurol 2019; 10 : 562 Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sj?gren Syndrome.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399