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DNAJB7 antibody

Anti-DNAJB7 antibody used in IHC (Paraffin sections) (IHC-P). GTX123260
Anti-DNAJB7 antibody used in Western Blot (WB). GTX123260

Cat. No. GTX123260

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DNAJB7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

NT2D1 , SK-N-SH

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJB7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member B7 , DJ5 , HSC3

Background

DNAJB7 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DNAJB7 antibody used in IHC (Paraffin sections) (IHC-P). GTX123260

GTX123260 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using DNAJB7(GTX123260) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNAJB7 antibody used in Western Blot (WB). GTX123260

GTX123260 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: SK-N-SH
10% SDS PAGE
GTX123260 diluted at 1:3000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169