Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNAJC1 antibody [C1C3] (GTX103858) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using DNAJC1(GTX103858) antibody at 1:200 dilution.