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DNAJC1 antibody [C1C3]

Anti-DNAJC1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103858
Anti-DNAJC1 antibody [C1C3] used in Western Blot (WB). GTX103858

Cat No. GTX103858

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member C1 , DNAJL1 , ERdj1 , HTJ1 , MTJ1

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein , Nucleus membrane; Single-pass type I membrane protein , Microsome membrane; Single-pass type I membrane protein , Endoplasmic reticulum membrane , Nucleus membrane , Microsome membrane

Background

May modulate protein synthesis.

Database

Research Area

DATA IMAGES
Anti-DNAJC1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103858

GTX103858 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DNAJC1(GTX103858) antibody at 1:200 dilution.

Anti-DNAJC1 antibody [C1C3] used in Western Blot (WB). GTX103858

GTX103858 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX103858 diluted at 1:1000

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf