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DNAJC1 antibody [C1C3]

Anti-DNAJC1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103858
Anti-DNAJC1 antibody [C1C3] used in Western Blot (WB). GTX103858

Cat. No. GTX103858

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all DNAJC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950129

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member C1 , DNAJL1 , ERdj1 , HTJ1 , MTJ1

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein , Nucleus membrane; Single-pass type I membrane protein , Microsome membrane; Single-pass type I membrane protein , Endoplasmic reticulum membrane , Nucleus membrane , Microsome membrane

Background

May modulate protein synthesis.

Database

Research Area

DATA IMAGES

Anti-DNAJC1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103858

GTX103858 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DNAJC1(GTX103858) antibody at 1:200 dilution.

Anti-DNAJC1 antibody [C1C3] used in Western Blot (WB). GTX103858

GTX103858 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNAJC1 antibody [C1C3] (GTX103858) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Y Wu et al. Metabolites 2024; 14 (1) : 51 Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response
Application : WB
Reactivity : Human
Tsai YL et al. J Biol Chem 2015; 290 (13) : 8049-64 Characterization and mechanism of stress-induced translocation of 78-kilodalton glucose-regulated protein (GRP78) to the cell surface.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169