GeneTex
United States (US)

DNAJC7 antibody [N2C3]

GTX104079_IFA_19011506_304.jpg
GTX104079_39742_IHC_19011506_863.jpg
GTX104079_39742_WB_M_brain_19011506_211.jpg
GTX104079_WB_19011506_480.jpg

Cat No. GTX104079

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431 , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNAJC7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dnaj Heat Shock Protein Family (Hsp40) Member C7,Dj11,Djc7,Tpr2,Ttc2,Dnajc7

Background

DNAJC7 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]

Database

DATA IMAGES
GTX104079_IFA_19011506_304.jpg

GTX104079 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNAJC7(GTX104079) antibody at 1:200 dilution.

GTX104079_39742_IHC_19011506_863.jpg

GTX104079 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DNAJC7(GTX104079) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104079_39742_WB_M_brain_19011506_211.jpg

GTX104079 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX104079 diluted at 1:1000

GTX104079_WB_19011506_480.jpg

GTX104079 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
7.5% SDS PAGE
GTX104079 diluted at 1:1500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169