Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNALI1 antibody (GTX116217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

DNALI1 antibody detects DNALI1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DNALI1 antibody (GTX116217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of methanol-fixed A549, using DNALI1(GTX116217) antibody at 1:500 dilution.

DNALI1 antibody detects DNALI1 protein at cytosol on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
DNALI1 antibody (GTX116217) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

DNALI1 antibody detects DNALI1 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
DNALI1 antibody (GTX116217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min