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DNMT1 antibody [60B1220.1]

Anti-DNMT1 antibody [60B1220.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48548
Anti-DNMT1 antibody [60B1220.1] used in Western Blot (WB). GTX48548
Anti-DNMT1 antibody [60B1220.1] used in Western Blot (WB). GTX48548
Anti-DNMT1 antibody [60B1220.1] used in Flow cytometry (FACS). GTX48548
Anti-DNMT1 antibody [60B1220.1] used in IHC (Paraffin sections) (IHC-P). GTX48548

Cat No. GTX48548

Host

Mouse

Clonality

Monoclonal

Clone Name

60B1220.1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, IP, ChIP assay, MS, IHC (Free Floating)

Reactivity

Human, Mouse, Rat, Pig
Package
50 μg ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 - 0.5 μg/ml
ICC/IF 1:10
IHC-P 1:50 - 1:500
FACS Assay dependent
IP 1:10 - 1:500
ChIP assay 1:20 - 1:1000
MS Assay dependent
IHC (Free Floating) 1:500
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI

Cellular Localization

Nucleus

Background

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT1 antibody [60B1220.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48548

GTX48548 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1].
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10

Anti-DNMT1 antibody [60B1220.1] used in Western Blot (WB). GTX48548

GTX48548 WB Image

WB analysis of HeLa cell lysate using GTX48548 DNMT1 antibody [60B1220.1].

Anti-DNMT1 antibody [60B1220.1] used in Western Blot (WB). GTX48548

GTX48548 WB Image

WB analysis of 2102EP cells / human DNMT1 recombinant protein / NIH3T3 cells / D3 cells in the (A)/(C)/(E)/(G) absence and (B)/(D)/(F)/(H) presence of immunizing peptide using GTX48548 DNMT1 antibody [60B1220.1].
Dilution : 5 μg/ml for cells / 0.1μg/ml for recombinant protein

Anti-DNMT1 antibody [60B1220.1] used in Flow cytometry (FACS). GTX48548

GTX48548 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1].
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA

Anti-DNMT1 antibody [60B1220.1] used in IHC (Paraffin sections) (IHC-P). GTX48548

GTX48548 IHC-P Image

IHC-P analysis of human hepatocellular carcinoma tissue using GTX48548 DNMT1 antibody [60B1220.1].
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf