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GeneTex
United States (US)

DNMT1 antibody [N1], N-term

KO/KD
GTX116011_42732_20180316_WB_KO_watermark_18121410_274.jpg
GTX116011_40135_20150612_IHC_18121410_824.jpg
GTX116011_40135_ChIP_18121410_708.jpg
GTX116011_42732_20170209_WB_18121410_434.jpg
GTX116011_42732_20170330_WB_18121410_770.jpg
GTX116011_40135_IP_18121410_363.jpg
GTX116011_42732_20170503_IFA_18121410_168.jpg

Cat No. GTX116011

Reference 14
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human
See all DNMT1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , NCI-H929
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human DNMT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Methyltransferase 1,Adcadn,Aim,Cxxc9,Dnmt,Hsn1E,Mcmt,M.Hsai,Dnmt1

Cellular Localization

Nucleus

Background

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX116011_42732_20180316_WB_KO_watermark_18121410_274.jpg

GTX116011 WB Image

Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX116011_40135_20150612_IHC_18121410_824.jpg

GTX116011 IHC-P Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX116011_40135_ChIP_18121410_708.jpg

GTX116011 ChIP assay Image

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2.
ChIP experiment and primer designs are based on Cancer Res. 2009 Sep 15;69(18):7412-21.

GTX116011_42732_20170209_WB_18121410_434.jpg

GTX116011 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.
GTX116011_42732_20170330_WB_18121410_770.jpg

GTX116011 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX116011_40135_IP_18121410_363.jpg

GTX116011 IP Image

DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term (GTX116011)
5 % SDS-PAGE
The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
GTX116011_42732_20170503_IFA_18121410_168.jpg

GTX116011 ICC/IF Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
REFERENCE

Application Reference

Huang MH et al. Pharmacol Res 2018; 139 : 50-61 Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.
Application : WB
Reactivity : Human
Weng YP et al. Oncotarget 2018; 9 (1) : 361-374 The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Application : WB
Reactivity : Human
Chen YJ et al. J Virol 2017; (Epub ahead of print) Epstein-Barr Virus Rta-Mediated Accumulation of DNA Methylation Interferes with CTCF Binding in Both Host and Viral Genomes.
Application : ChIP assay
Reactivity : Human
Sharma S et al. J Biol Chem 2016; 291 (37) : 19351-63 Secreted Protein Acidic and Rich in Cysteine (SPARC) Mediates Metastatic Dormancy of Prostate Cancer in Bone.
Application : WB
Li HK et al. Sci Rep 2016; 6 : 28637 DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
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Submit a Reference
Application Reference
Huang MH et al. Pharmacol Res 2018; 139:50-61 Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.
Application : WB
Reactivity : Human
Weng YP et al. Oncotarget 2018; 9 (1):361-374 The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Application : WB
Reactivity : Human
Chen YJ et al. J Virol 2017; (Epub ahead of print) Epstein-Barr Virus Rta-Mediated Accumulation of DNA Methylation Interferes with CTCF Binding in Both Host and Viral Genomes.
Application : ChIP assay
Reactivity : Human
Sharma S et al. J Biol Chem 2016; 291 (37):19351-63 Secreted Protein Acidic and Rich in Cysteine (SPARC) Mediates Metastatic Dormancy of Prostate Cancer in Bone.
Application : WB
Li HK et al. Sci Rep 2016; 6:28637 DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Yao-Yu H et al. Oncotarget 2016; (Epub ahead of print) Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.
Application : WB
Reactivity : Human
Wijenayake S et al. J Comp Physiol B 2016; 186 (3):333-42 The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans).
Application : WB
Reactivity : Human
Wen HC et al. PLoS One 2015; 10 (4):e0125518 Elevation of soluble guanylate cyclase suppresses proliferation and survival of human breast cancer cells.
Application : WB
Reactivity : Human
Aslanian A et al. DNA Repair (Amst) 2014; 15 (Epub Ahead of Print):29-38 Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells.
Application : WB
Reactivity : Human
Wang SC et al. J Agric Food Chem 2014; Antroquinonol D, isolated from Antrodia camphorate, with DNA demethylation and anti-cancer potential.
Application : WB
Reactivity : Human
Lin YC et al. J Cell Sci 2014; 127 (Pt 1):85-100 Tumor suppressor SCUBE2 inhibits breast-cancer cell migration and invasion through the reversal of epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Xu X et al. Diagn Pathol 2012; 7:33 The expression patterns and correlations of claudin-6, methy-CpG binding protein 2, DNA methyltransferase 1, histone deacetylase 1, acetyl-histone H3 and acetyl-histone H4 and their clinicopathological significance in breast invasive ductal carcinomas.
Application : IHC-P
Reactivity : Human
Li CT et al. J Cell Biochem 2011; 112 (10):3044-53 Vorinostat, SAHA, represses telomerase activity via epigenetic regulation of telomerase reverse transcriptase in non-small cell lung cancer cells.
Application : ChIP assay
Reactivity : Human
Tang SC et al. Cancer 2011; 117 (14):3209-21 Epigenetic mechanisms for silencing glutathione S-transferase m2 expression by hypermethylated specificity protein 1 binding in lung cancer.
Application : ChIP assay
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169