DNMT1 antibody [N1], N-term

Cat No. GTX116011

Reference 16

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity	Human
Package	100 μl ($329), 25 μl ($169)

See all DNMT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:2000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , NCI-H929

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human DNMT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI

Cellular Localization

Nucleus

Background

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 1786 DNMT1
UniProt: P26358 DNMT1

Research Area

DATA IMAGES

GTX116011 WB Image

Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116011 IHC-P Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX116011 ChIP assay Image

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2.
ChIP experiment and primer designs are based on Cancer Res. 2009 Sep 15;69(18):7412-21.

GTX116011 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.

GTX116011 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116011 IP Image

DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term (GTX116011)
5 % SDS-PAGE
The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX116011 ICC/IF Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.