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DNMT1 antibody [N1], N-term

KO/KD Orthogonal
Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011
Anti-DNMT1 antibody [N1], N-term used in ChIP assay (ChIP assay). GTX116011
Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Anti-DNMT1 antibody [N1], N-term used in Immunoprecipitation (IP). GTX116011
Anti-DNMT1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116011
Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011
Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011

Cat No. GTX116011

Reference 16
6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all DNMT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , NCI-H929

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human DNMT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036782

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI

Cellular Localization

Nucleus

Background

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011

GTX116011 WB Image

KO/KD

Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011

GTX116011 IHC-P Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNMT1 antibody [N1], N-term used in ChIP assay (ChIP assay). GTX116011

GTX116011 ChIP assay Image

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2.
ChIP experiment and primer designs are based on Cancer Res. 2009 Sep 15;69(18):7412-21.

Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011

GTX116011 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.

Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011

GTX116011 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT1 antibody [N1], N-term used in Immunoprecipitation (IP). GTX116011

GTX116011 IP Image

DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term (GTX116011)
5 % SDS-PAGE
The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-DNMT1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116011

GTX116011 ICC/IF Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011

GTX116011 IHC-P Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011

GTX116011 IHC-P Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Li H et al. Onco Targets Ther 2020; 13 : 1211-1223 Transcriptomics Analysis of the Tumor-Inhibitory Pathways of 6-Thioguanine in MCF-7 Cells via Silencing DNMT1 Activity.
Application : WB
Reactivity : Human
Wei GJ et al. AAPS J 2019; 21 (5) : 86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
Application : WB
Reactivity : Human
Huang MH et al. Pharmacol Res 2018; 139 : 50-61 Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.
Application : WB
Reactivity : Human
Weng YP et al. Oncotarget 2018; 9 (1) : 361-374 The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Application : WB
Reactivity : Human
Chen YJ et al. J Virol 2017; (Epub) Epstein-Barr Virus Rta-Mediated Accumulation of DNA Methylation Interferes with CTCF Binding in Both Host and Viral Genomes.
Application : ChIP assay
Reactivity : Human
Show More +
Submit a Reference

REVIEW

DNMT1 antibody [N1], N-term Cat No. GTX116011

Rating
( Average 5 based on 6 users reviews)
Application
Western Blot(WB)
( Average 5 based on 4 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Diagn Pathol in 2012.PMID: 22455563
Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Diagn Pathol in 2012.PMID: 22455563
Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 27344963
Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25928539
Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018.PMID: 29416619
Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018.PMID: 29416619
Show More +
Submit a Review
Application Reference
Li H et al. Onco Targets Ther 2020; 13:1211-1223 Transcriptomics Analysis of the Tumor-Inhibitory Pathways of 6-Thioguanine in MCF-7 Cells via Silencing DNMT1 Activity.
Application : WB
Reactivity : Human
Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
Application : WB
Reactivity : Human
Huang MH et al. Pharmacol Res 2018; 139:50-61 Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.
Application : WB
Reactivity : Human
Weng YP et al. Oncotarget 2018; 9 (1):361-374 The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Application : WB
Reactivity : Human
Chen YJ et al. J Virol 2017; (Epub) Epstein-Barr Virus Rta-Mediated Accumulation of DNA Methylation Interferes with CTCF Binding in Both Host and Viral Genomes.
Application : ChIP assay
Reactivity : Human
Sharma S et al. J Biol Chem 2016; 291 (37):19351-63 Secreted Protein Acidic and Rich in Cysteine (SPARC) Mediates Metastatic Dormancy of Prostate Cancer in Bone.
Application : WB
Li HK et al. Sci Rep 2016; 6:28637 DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Yao-Yu H et al. Oncotarget 2016; (Epub) Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.
Application : WB
Reactivity : Human
Wijenayake S et al. J Comp Physiol B 2016; 186 (3):333-42 The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans).
Application : WB
Reactivity : Human
Wen HC et al. PLoS One 2015; 10 (4):e0125518 Elevation of soluble guanylate cyclase suppresses proliferation and survival of human breast cancer cells.
Application : WB
Reactivity : Human
Aslanian A et al. DNA Repair (Amst) 2014; 15 (Epub):29-38 Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells.
Application : WB
Reactivity : Human
Wang SC et al. J Agric Food Chem 2014; Antroquinonol D, isolated from Antrodia camphorate, with DNA demethylation and anti-cancer potential.
Application : WB
Reactivity : Human
Lin YC et al. J Cell Sci 2014; 127 (Pt 1):85-100 Tumor suppressor SCUBE2 inhibits breast-cancer cell migration and invasion through the reversal of epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Xu X et al. Diagn Pathol 2012; 7:33 The expression patterns and correlations of claudin-6, methy-CpG binding protein 2, DNA methyltransferase 1, histone deacetylase 1, acetyl-histone H3 and acetyl-histone H4 and their clinicopathological significance in breast invasive ductal carcinomas.
Application : IHC-P
Reactivity : Human
Li CT et al. J Cell Biochem 2011; 112 (10):3044-53 Vorinostat, SAHA, represses telomerase activity via epigenetic regulation of telomerase reverse transcriptase in non-small cell lung cancer cells.
Application : ChIP assay
Reactivity : Human
Tang SC et al. Cancer 2011; 117 (14):3209-21 Epigenetic mechanisms for silencing glutathione S-transferase m2 expression by hypermethylated specificity protein 1 binding in lung cancer.
Application : ChIP assay
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169