APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.2-1 μg /ml |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Highly purified recombinant mouse Dnmt1 (amino acids 1-248), soluble form
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DNA methyltransferase (cytosine-5) 1 , Cxxc9 , Dnmt , Dnmt1o , MCMT , MTase , Met-1 , Met1 , MommeD2 , m.MmuI
Cellular Localization
Nucleus,Cytoplasm
Background
This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
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GTX00734 WB Image
WB analysis of various samples using GTX00734 DNMT1 antibody.
Lane 1 : Mouse ES cell line J1 (Dnmt1 +/+)
Lane 2 : Mouse ES cell line 36 (Dnmt1 -/-)
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GTX00734 ELISA Image
ELISA analysis of GST-Dnmt3 (1-248) fusion protein using GTX00734 DNMT1 antibody.
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GTX00734 IP Image
IP analysis of mouse MEL cells using GTX00734 DNMT1 antibody.
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GTX00734 ICC/IF Image
ICC/IF analysis of Mouse embryonic stem cells (E14) using GTX00734 DNMT1 antibody.
Nuclei were stained with DAPI.
Dilution : 1:5000
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GTX00734 WB Image
WB analysis of various samples using GTX00734 DNMT1 antibody.
Lane 1 : Immunogen: GST-Dnmt3 (1-248) fusion protein
Lane 2 : NIH3T3 whole cell lysate (20 μg)
Dilution : 1 μg/ml
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REFERENCE
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REVIEW
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