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DNMT1 antibody

Anti-DNMT1 antibody used in Immunoprecipitation (IP). GTX00734
Anti-DNMT1 antibody used in Western Blot (WB). GTX00734
Anti-DNMT1 antibody used in Western Blot (WB). GTX00734
Anti-DNMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00734
Anti-DNMT1 antibody used in ELISA (ELISA). GTX00734

Cat No. GTX00734

Host

Rabbit

Clonality

Polyclonal

Application

WB, ICC/IF, IP, ELISA, ChIP assay

Reactivity

Human, Mouse
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-1 μg /ml
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4), 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Highly purified recombinant mouse Dnmt1 (amino acids 1-248), soluble form

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA methyltransferase (cytosine-5) 1 , Cxxc9 , Dnmt , Dnmt1o , MCMT , MTase , Met-1 , Met1 , MommeD2 , m.MmuI

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-DNMT1 antibody used in Immunoprecipitation (IP). GTX00734

GTX00734 IP Image

IP analysis of mouse MEL cells using GTX00734 DNMT1 antibody.

Anti-DNMT1 antibody used in Western Blot (WB). GTX00734

GTX00734 WB Image

WB analysis of various samples using GTX00734 DNMT1 antibody.
Lane 1 : Immunogen: GST-Dnmt3 (1-248) fusion protein
Lane 2 : NIH3T3 whole cell lysate (20 μg)
Dilution : 1 μg/ml

Anti-DNMT1 antibody used in Western Blot (WB). GTX00734

GTX00734 WB Image

WB analysis of various samples using GTX00734 DNMT1 antibody.
Lane 1 : Mouse ES cell line J1 (Dnmt1 +/+)
Lane 2 : Mouse ES cell line 36 (Dnmt1 -/-)

Anti-DNMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00734

GTX00734 ICC/IF Image

ICC/IF analysis of Mouse embryonic stem cells (E14) using GTX00734 DNMT1 antibody.
Nuclei were stained with DAPI.
Dilution : 1:5000

Anti-DNMT1 antibody used in ELISA (ELISA). GTX00734

GTX00734 ELISA Image

ELISA analysis of GST-Dnmt3 (1-248) fusion protein using GTX00734 DNMT1 antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 319