GeneTex
United States (US)

DNMT1 antibody

Orthogonal
GTX30364_204_ICC-IF_18121410_615.jpg
GTX30364_1393_WB_18121410_626.jpg
GTX30364_549_IHC-P_18121410_109.jpg
GTX30364_1392_WB_18121410_451.jpg
GTX30364_548_IHC-P_18121410_629.jpg

Cat No. GTX30364

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50 - 1:200
IHC-P 1:100
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

183 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Contained within amino acids 1-125 of the N-terminus of human Dnmt1 [UniProt# P26358]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Methyltransferase 1,Adcadn,Aim,Cxxc9,Dnmt,Hsn1E,Mcmt,M.Hsai,Dnmt1

Cellular Localization

Nucleus

Background

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30364_204_ICC-IF_18121410_615.jpg

GTX30364 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30364 DNMT1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:50

GTX30364_1393_WB_18121410_626.jpg

GTX30364 WB Image

WB analysis of HEK293 cell lysate using GTX30364 DNMT1 antibody.

GTX30364_549_IHC-P_18121410_109.jpg

GTX30364 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX30364 DNMT1 antibody.

GTX30364_1392_WB_18121410_451.jpg

GTX30364 WB Image

Orthogonal

WB analysis of T24 cell lysate treated with 0.5 uM 5-Aza-Deoxycytidine using GTX30364 DNMT1 antibody.
Loading : 50μg
Lane 1 : untreated T24 degrees cells
Lane 2 : cell lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days

GTX30364_548_IHC-P_18121410_629.jpg

GTX30364 IHC-P Image

IHC-P analysis of mouse small intestine tissue using GTX30364 DNMT1 antibody.

REFERENCE
Package List Price ($)
$ 329