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DNMT3A antibody

KO/KD
GTX129125_41668_20160428_WB_shRNA_watermark_18121410_153.jpg
GTX129125_41668_IHC_M_18121410_922.jpg
GTX129125_41668_IP_18121410_155.jpg
GTX129125_41668_20170929_WB_18121410_223.jpg
GTX129125_41668_IP_2_18121410_272.jpg
GTX129125_41668_IFA_18121410_235.jpg
GTX129125_41668_20170915_WB_R_18121410_839.jpg
GTX129125_41668_20170915_WB_M_18121410_507.jpg

Cat No. GTX129125

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, EM
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
EM Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Neuro2A , PC-12 , Rat2 , *CL1-0

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DNA (cytosine-5-)-methyltransferase 3 alpha Antibody , DNMT3A2 Antibody , M.HsaIIIA Antibody

Cellular Localization

Nucleus , Cytoplasm

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX129125_41668_20160428_WB_shRNA_watermark_18121410_153.jpg

GTX129125 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129125_41668_IHC_M_18121410_922.jpg

GTX129125 IHC-P Image

DNMT3A antibody detects DNMT3A protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
DNMT3A antibody (GTX129125) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX129125_41668_IP_18121410_155.jpg

GTX129125 IP Image

DNMT3A antibody immunoprecipitates DNMT3A protein in IP experiments.
IP samples: 293T nuclear extract
A. 50 μg 293T nuclear extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of DNMT3A protein by 4 μg DNMT3A antibody (GTX129125)
5 % SDS-PAGE
The immunoprecipitated DNMT3A protein was detected by DNMT3A antibody (GTX129125) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX129125_41668_20170929_WB_18121410_223.jpg

GTX129125 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129125_41668_IP_2_18121410_272.jpg

GTX129125 IP Image

Immunoprecipitation of DNMT3A protein from 293T whole cell extracts using 5 μg of DNMT3A antibody (GTX129125) or DNMT3A antibody (GTX129126).
Western blot analysis was performed using DNMT3A antibody (GTX129125).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX129125_41668_IFA_18121410_235.jpg

GTX129125 ICC/IF Image

DNMT3A antibody detects DNMT3A protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT3A protein stained by DNMT3A antibody (GTX129125) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX129125_41668_20170915_WB_R_18121410_839.jpg

GTX129125 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129125_41668_20170915_WB_M_18121410_507.jpg

GTX129125 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

DNMT3A antibody Cat No. GTX129125

Rating ( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Electron microscopy(EM) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 27-Mar-2017
Score :
Application Tested : EM
Sample Species : Ms
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:5, 4°C, overnightHr
Date : Anonymous submitted on 07-Jun-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : NIH/3T3 cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 26-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-0 cells
Amount used : 50μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:1000, 37°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169