GeneTex
United States (US)

DNMT3A antibody

GTX30365_321_IHC-P_18121410_647.jpg
GTX30365_205_ICC-IF_18121410_482.jpg
GTX30365_1394_WB_18121410_687.jpg

Cat No. GTX30365

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:500
IHC-P 1:200
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

102 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Serum, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 10-118 of human Dnmt3a [UniProt# Q9Y6K1]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DNMT3A2 antibody , M.HsaIIIA antibody , TBRS antibody

Cellular Localization

Nucleus,Cytoplasm

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30365_321_IHC-P_18121410_647.jpg

GTX30365 IHC-P Image

IHC-P analysis of human testis tissue using GTX30365 DNMT3A antibody.

GTX30365_205_ICC-IF_18121410_482.jpg

GTX30365 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX30365 DNMT3A antibody.
Green : primary antibody
Red : beta-Tubulin
Blue : DAPI

GTX30365_1394_WB_18121410_687.jpg

GTX30365 WB Image

WB analysis of HCT116 mock (Lane 1) or overexpressing DNMT3A (Lane 2) nuclear extracts using GTX30365 DNMT3A antibody.
Loading : 40μg

REFERENCE
Package List Price ($)
$ 339