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DNMT3A antibody

Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX30365
Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30365
Anti-DNMT3A antibody used in Western Blot (WB). GTX30365

Cat No. GTX30365

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:500
IHC-P 1:200
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Amino acids 10-118 of human Dnmt3a [UniProt# Q9Y6K1]

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385509

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA methyltransferase 3 alpha , DNMT3A2 , M.HsaIIIA , TBRS

Cellular Localization

Nucleus,Cytoplasm

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX30365

GTX30365 IHC-P Image

IHC-P analysis of human testis tissue using GTX30365 DNMT3A antibody.

Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30365

GTX30365 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX30365 DNMT3A antibody.
Green : primary antibody
Red : beta-Tubulin
Blue : DAPI

Anti-DNMT3A antibody used in Western Blot (WB). GTX30365

GTX30365 WB Image

WB analysis of HCT116 mock (Lane 1) or overexpressing DNMT3A (Lane 2) nuclear extracts using GTX30365 DNMT3A antibody.
Loading : 40μg

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf