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DNMT3A antibody

Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30365
Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX30365
Anti-DNMT3A antibody used in Western Blot (WB). GTX30365

Cat. No. GTX30365

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all DNMT3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:500
IHC-P 1:200
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Amino acids 10-118 of human Dnmt3a [UniProt# Q9Y6K1]

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385509

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3 alpha , DNMT3A2 , M.HsaIIIA , TBRS

Cellular Localization

Nucleus,Cytoplasm

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30365

GTX30365 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX30365 DNMT3A antibody.
Green : primary antibody
Red : beta-Tubulin
Blue : DAPI

Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX30365

GTX30365 IHC-P Image

IHC-P analysis of human testis tissue using GTX30365 DNMT3A antibody.

Anti-DNMT3A antibody used in Western Blot (WB). GTX30365

GTX30365 WB Image

WB analysis of HCT116 mock (Lane 1) or overexpressing DNMT3A (Lane 2) nuclear extracts using GTX30365 DNMT3A antibody.
Loading : 40μg

REFERENCE

Application Reference

Ratovitski EA et al. Cell Cycle 2014; 13 (5) : 749-61 Phospho-ΔNp63α/microRNA network modulates epigenetic regulatory enzymes in squamous cell carcinomas.

Clone Reference

Robert MF et al. Nat Genet 2003; 33 (1):22402 DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells.
Beaulieu N et al. J Biol Chem 2002; 277 (31):28176-81 An essential role for DNA methyltransferase DNMT3B in cancer cell survival.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419