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DNMT3A antibody

Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33161
Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33161
Anti-DNMT3A antibody used in Western Blot (WB). GTX33161
Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33161
Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33161

Cat. No. GTX33161

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DNMT3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human DNMT3A

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3 alpha , DNMT3A2 , M.HsaIIIA , TBRS

Cellular Localization

Nucleus,Cytoplasm

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33161

GTX33161 ICC/IF Image

ICC/IF analysis of A549 cells using GTX33161 DNMT3A antibody.

Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33161

GTX33161 IHC-P Image

IHC-P analysis of human kidney tissue using GTX33161 DNMT3A antibody.
Dilution : 1:100

Anti-DNMT3A antibody used in Western Blot (WB). GTX33161

GTX33161 WB Image

WB analysis of various sample lysates using GTX33161 DNMT3A antibody.
Loading : 25μg per lane

Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33161

GTX33161 IHC-P Image

IHC-P analysis of rat brain tissue using GTX33161 DNMT3A antibody.
Dilution : 1:100

Anti-DNMT3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33161

GTX33161 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX33161 DNMT3A antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399