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DNMT3A antibody

Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55596
Anti-DNMT3A antibody used in Western Blot (WB). GTX55596
Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX55596

Cat. No. GTX55596

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human DNMT3A (NP_715640.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3 alpha , DNMT3A2 , M.HsaIIIA , TBRS

Cellular Localization

Nucleus,Cytoplasm

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-DNMT3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55596

GTX55596 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55596 DNMT3A antibody.

Anti-DNMT3A antibody used in Western Blot (WB). GTX55596

GTX55596 WB Image

WB analysis of various sample lysates using GTX55596 DNMT3A antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX55596

GTX55596 IP Image

IP analysis of 293T cell lysate using GTX55596 DNMT3A antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399