DNMT3B antibody

Cat. No. GTX125917

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Mouse
Package	100 μl ($399), 25 μl ($169)

See all DNMT3B products

Datasheet
Document

View product citations for antibody GTX125917 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

DNMT3B transfected 293T

Predict Reactivity

Human, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse DNMT3B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178364

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3B , MmuIIIB

Cellular Localization

Nucleus

Background

Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

Database

Gene ID: 13436 Dnmt3b
UniProt: O88509 Dnmt3b

Research Area

DATA IMAGES

GTX125917 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: DNMT3B transfected 293T lysates
7.5% SDS PAGE
GTX125917 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty