GeneTex
United States (US)

DNMT3B antibody

GTX125917_40842_WB_B_18121410_426.jpg

Cat No. GTX125917

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

Recombinant DNMT3B protein

Predict Reactivity

Human, Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse DNMT3B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Methyltransferase 3B,Mmuiiib,Dnmt3B

Cellular Localization

Nucleus

Background

Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

Database

Research Area

DATA IMAGES
GTX125917_40842_WB_B_18121410_426.jpg

GTX125917 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates 
B: DNMT3B transfected 293T lysates
7.5% SDS PAGE
GTX125917 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf