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DNMT3L antibody [N1C1]

Anti-DNMT3L antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115985
Anti-DNMT3L antibody [N1C1] used in Western Blot (WB). GTX115985

Cat No. GTX115985

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

NT2D1

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNMT3L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621537

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA methyltransferase 3 like

Cellular Localization

Nucleus

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT3L antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115985

GTX115985 ICC/IF Image

DNMT3L antibody [N1C1] detects DNMT3L protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT3L protein stained by DNMT3L antibody [N1C1] (GTX115985) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-DNMT3L antibody [N1C1] used in Western Blot (WB). GTX115985

GTX115985 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX115985 diluted at 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169