DNMT3L antibody

Cat. No. GTX23493
Cat. No.  GTX23493
Anti-DNMT3L antibody used in Immunoprecipitation (IP). GTX23493
GTX23493 IP Image

Affinity Purified Rabbit anti-DNMT3L was used at a 1:1,500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody. Transfection of U2OS cells (10cm dish) was accomplished using 3 μg of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For IP and WB conditions see Fuks et al. (2000). For western blotting the antibody was used at 0.4μg/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL. Note: this antibody works for western blotting only following immunoprecipitation. We have not had good success using the antibody directly for western blotting. The expected molecular weight of human DNMT3L is 43.6 kDa.

1 / 2
Anti-DNMT3L antibody used in Immunohistochemistry (IHC). GTX23493
GTX23493 IHC Image

Immunohistochemistry Rabbit Anti-Human DNMT3L Antibody 1:500

2 / 2
Anti-DNMT3L antibody used in Immunoprecipitation (IP). GTX23493
Anti-DNMT3L antibody used in Immunohistochemistry (IHC). GTX23493
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB IHC-P IP ELISA IHC
  • Reactivity
    Human
See all DNMT3L products