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DNMT3L antibody

Anti-DNMT3L antibody used in IHC (Paraffin sections) (IHC-P). GTX32565
Anti-DNMT3L antibody used in IHC (Paraffin sections) (IHC-P). GTX32565
Anti-DNMT3L antibody used in IHC (Paraffin sections) (IHC-P). GTX32565
Anti-DNMT3L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32565

Cat. No. GTX32565

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DNMT3L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:20 - 1:50
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-386 of human DNMT3L (NP_787063.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3 like

Cellular Localization

Nucleus

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-DNMT3L antibody used in IHC (Paraffin sections) (IHC-P). GTX32565

GTX32565 IHC-P Image

IHC-P analysis of rat testis tissue using GTX32565 DNMT3L antibody.
Dilution : 1:100

Anti-DNMT3L antibody used in IHC (Paraffin sections) (IHC-P). GTX32565

GTX32565 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32565 DNMT3L antibody.
Dilution : 1:100

Anti-DNMT3L antibody used in IHC (Paraffin sections) (IHC-P). GTX32565

GTX32565 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX32565 DNMT3L antibody.
Dilution : 1:100

Anti-DNMT3L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32565

GTX32565 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32565 DNMT3L antibody.

REFERENCE

Application Reference

Williamson SM et al. J Insect Physiol 2021; 134 : 104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399