Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNase I antibody [N1C3] (GTX109306) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DNase I antibody [N1C3] detects DNase I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
DNase I stained by DNase I antibody [N1C3] (GTX109306) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
DNase I antibody [N1C3] (GTX109306) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

DNase I antibody [N1C3] detects DNase I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DNase I protein stained by DNase I antibody [N1C3] (GTX109306) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
DNase I antibody [N1C3] (GTX109306) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min