Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DNase IL3 antibody [N3C3] detects DNase IL3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNase IL3 stained by DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.