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DNase IL3 antibody [N3C3]

Anti-DNase IL3 antibody [N3C3] used in Western Blot (WB). GTX114363
Anti-DNase IL3 antibody [N3C3] used in Western Blot (WB). GTX114363
Anti-DNase IL3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114363

Cat No. GTX114363

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DNase IL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620330

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

deoxyribonuclease 1 like 3 , DHP2 , DNAS1L3 , LSD , SLEB16

Background

This gene encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNase IL3 antibody [N3C3] used in Western Blot (WB). GTX114363

GTX114363 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNase IL3 antibody [N3C3] used in Western Blot (WB). GTX114363

GTX114363 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNase IL3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114363

GTX114363 ICC/IF Image

DNase IL3 antibody [N3C3] detects DNase IL3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNase IL3 stained by DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE

There are currently no references for DNase IL3 antibody [N3C3] (GTX114363). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for DNase IL3 antibody [N3C3] (GTX114363). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169