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DOCK1 antibody

Anti-DOCK1 antibody used in Western Blot (WB). GTX100243
Anti-DOCK1 antibody used in Western Blot (WB). GTX100243
Anti-DOCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100243
Anti-DOCK1 antibody used in Western Blot (WB). GTX100243
Anti-DOCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100243

Cat. No. GTX100243

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DOCK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

215 kDa. ( Note )

Positive Control

293T , HeLaS3 , C8D30 , NIH-3T3 , Raw264.7 , C2C12

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DOCK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240720

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dedicator of cytokinesis 1 , DOCK180 , ced5

Background

This gene product binds to the SH3 domain of CRK protein. It may regulate cell surface extension and may have a role in the cell surface extension of an engulfing cell around a dying cell during apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DOCK1 antibody used in Western Blot (WB). GTX100243

GTX100243 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DOCK1 antibody (GTX100243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DOCK1 antibody used in Western Blot (WB). GTX100243

GTX100243 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DOCK1 antibody (GTX100243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DOCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100243

GTX100243 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using DOCK1/180(GTX100243) antibody at 1:200 dilution.

Anti-DOCK1 antibody used in Western Blot (WB). GTX100243

GTX100243 WB Image

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX100243 diluted at 1:1000

Anti-DOCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100243

GTX100243 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using DOCK 180 (GTX100243) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169