GeneTex
United States (US)

DOCK1 antibody

GTX100243_39456_20170630_WB_M_19011506_675.jpg
GTX100243_IHC_19011506_760.jpg
GTX100243_WB_19011506_473.jpg
GTX100243_39456_20170608_WB_R_19011506_525.jpg
GTX100243_39456_IFA_19011506_966.jpg

Cat No. GTX100243

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

215 kDa. ( Note )

Positive Control

293T , HeLaS3 , PC-12 , C8D30 , NIH-3T3 , Raw264.7 , C2C12
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DOCK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DOCK180 antibody, ced5 antibody, DOCK1 antibody, DOwnstream of CrK antibody, 180 kDa protein downstream of CRK antibody, dedicator of cytokinesis protein 1 antibody, dedicator of cyto-kinesis 1 antibody, dedicator of cytokinesis 1 antibody

Background

This gene product binds to the SH3 domain of CRK protein. It may regulate cell surface extension and may have a role in the cell surface extension of an engulfing cell around a dying cell during apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100243_39456_20170630_WB_M_19011506_675.jpg

GTX100243 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DOCK1 antibody (GTX100243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100243_IHC_19011506_760.jpg

GTX100243 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using DOCK 180 (GTX100243) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100243_WB_19011506_473.jpg

GTX100243 WB Image

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX100243 diluted at 1:1000

GTX100243_39456_20170608_WB_R_19011506_525.jpg

GTX100243 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with DOCK1 antibody (GTX100243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100243_39456_IFA_19011506_966.jpg

GTX100243 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using DOCK1/180(GTX100243) antibody at 1:200 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169