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DOK2 antibody [N1C1]

Anti-DOK2 antibody [N1C1] used in Western Blot (WB). GTX101577
Anti-DOK2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101577
Anti-DOK2 antibody [N1C1] used in Western Blot (WB). GTX101577

Cat No. GTX101577

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , Jurkat , Raji , NCI-H929

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DOK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

docking protein 2,p56DOK,p56dok-2

Background

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-DOK2 antibody [N1C1] used in Western Blot (WB). GTX101577

GTX101577 WB Image

DOK2 antibody [N1C1] detects DOK2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DOK2 antibody [N1C1] (GTX101577) at a dilution of 1:10000.

Anti-DOK2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101577

GTX101577 ICC/IF Image

DOK2 antibody [N1C1] detects DOK2 protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DOK2 protein stained by DOK2 antibody [N1C1] (GTX101577) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-DOK2 antibody [N1C1] used in Western Blot (WB). GTX101577

GTX101577 WB Image

DOK2 antibody detects DOK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DOK2 antibody (GTX101577) diluted at a dilution of 1:10000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169