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DOK2 (phospho Tyr299) antibody

Anti-DOK2 (phospho Tyr299) antibody used in IHC (Paraffin sections) (IHC-P). GTX50299
Anti-DOK2 (phospho Tyr299) antibody used in Western Blot (WB). GTX50299
Anti-DOK2 (phospho Tyr299) antibody used in Western Blot (WB). GTX50299
Anti-DOK2 (phospho Tyr299) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50299

Cat No. GTX50299

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y(p)-A-V) derived from human DOK2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

docking protein 2 , p56DOK , p56dok-2

Background

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DOK2 (phospho Tyr299) antibody used in IHC (Paraffin sections) (IHC-P). GTX50299

GTX50299 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50299 DOK2 (phospho Tyr299) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-DOK2 (phospho Tyr299) antibody used in Western Blot (WB). GTX50299

GTX50299 WB Image

WB analysis of extracts from JK cells treated with G-CSF or calf intestinal phosphatase (CIP) using GTX50299 DOK2 (phospho Tyr299) antibody.

Anti-DOK2 (phospho Tyr299) antibody used in Western Blot (WB). GTX50299

GTX50299 WB Image

WB analysis of extracts from Jurkat cells untreated or treated with insulin using GTX50299 DOK2 (phospho Tyr299) antibody.

Anti-DOK2 (phospho Tyr299) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50299

GTX50299 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50299 DOK2 (phospho Tyr299) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf