GeneTex
United States (US)

DOT1L antibody

GTX104736_39673_CT_WB_19011506_546.jpg

Cat No. GTX104736

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

185 kDa. ( Note )

Positive Control

*H1299 ,

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DOT1L. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dot1 Like Histone Lysine Methyltransferase,Dot1,Kmt4,Dot1L

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
GTX104736_39673_CT_WB_19011506_546.jpg

GTX104736 WB Image

DOT1L antibody detects DOT1L protein by Western blot analysis.
A. H1299 whole cell lysate/extract
% SDS-PAGE
DOT1L antibody (GTX104736) dilution: 1:1000

REVIEW

DOT1L antibody Cat No. GTX104736

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 06-May-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1299 cells
Blocking : 3% nonfat milk in Tris-buffered saline/Tween 20, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169