APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Kidney Tissue Lysate(GTX27936)
Product Note
At least four isoforms of DPAGT1 are known to exist; this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
DPAGT1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human DPAGT1.The immunogen is located within amino acids 30 - 80 of DPAGT1(NP_001373).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 , ALG7 , CDG-Ij , CDG1J , CMS13 , CMSTA2 , D11S366 , DGPT , DPAGT , DPAGT2 , G1PT , GPT , UAGT , UGAT
Background
The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
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GTX16275 IHC-P Image
IHC-P analysis of human kidney tissue using GTX16275 DPAGT1 antibody.
Working concentration : 20 μg/ml
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GTX16275 IHC-P Image
IHC-P analysis of human kidney tissue using GTX16275 DPAGT1 antibody.
Working concentration : 2.5 μg/ml
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GTX16275 WB Image
WB analysis of mouse kidney tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX16275 DPAGT1 antibody.
Working concentration : 1 μg/ml
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REFERENCE
There are currently no references for DPAGT1 antibody (GTX16275). Be the first to share your publications with this product.
REVIEW
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